FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3863552
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG63285316.38008236979857TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1016482.6309468592631857No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG433121.1210409488470714TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC354520.9176012125629472TruSeq Adapter, Index 9 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT325050.8413242529154519TruSeq Adapter, Index 9 (100% over 48bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291780.7552117843890803No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG199240.5156912602703418TruSeq Adapter, Index 9 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT177930.46053476179432806TruSeq Adapter, Index 9 (100% over 48bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160340.41500670885237206No Hit
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG154440.39973578717201164TruSeq Adapter, Index 9 (100% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC112160.2903028094354625TruSeq Adapter, Index 9 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT104220.26975177246223164TruSeq Adapter, Index 9 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT62830.1626223744367877TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG61790.1599305509541479TruSeq Adapter, Index 9 (97% over 49bp)
GGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG61450.15905053173866948TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATG60210.15584104989398356TruSeq Adapter, Index 9 (97% over 49bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC58160.15053505168301085TruSeq Adapter, Index 9 (100% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATG51800.13407351576994433TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATG49170.1272663083090379TruSeq Adapter, Index 9 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA48770.12623099158494566TruSeq Adapter, Index 9 (100% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC46340.11994144248608535TruSeq Adapter, Index 9 (100% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG45810.11856964782666314TruSeq Adapter, Index 9 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT44900.1162143022793533TruSeq Adapter, Index 9 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGT41320.1069482175987278Illumina Single End PCR Primer 1 (96% over 29bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC40660.10523994500397563TruSeq Adapter, Index 9 (97% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC40440.10467052080572489TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATG39990.10350578949112112TruSeq Adapter, Index 9 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG59600.066.4946061
GGGTCGG10100.064.472991
ACACTCT113450.064.248421
AAAAAGG815600.061.6667870
TGATCGG27900.061.611751
CACTCTT142250.055.729372
AGGGGGG42850.054.3321761
ACTCTTT146350.054.2623673
CACGGTC7100.053.73101832
GGTCGGA13350.052.958822
CACGAGC13150.052.16596632
TCGGGGG3350.051.1927264
CTTTCCC159150.050.755196
AAAAAAG1010250.050.22168769
TCTTTCC157900.050.1817325
TTTCCCT160950.050.0788427
TCCCTAC162100.049.9610639
CCCTACA162850.049.8384310
CCTACAC163550.049.6251211
CTCTTTC160500.049.5439074
Produced by FastQC (version 0.11.5)