FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6856378
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4363386.363972348082325TruSeq Adapter, Index 8 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1827472.6653577151084726No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG989021.4424817301496504TruSeq Adapter, Index 8 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560170.8170057135122947No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG405140.5908950760882787TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATG359770.5247231118237647TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATT348080.5076732933919337TruSeq Adapter, Index 8 (97% over 48bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269160.39256878777686993No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG239650.34952857033261586TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTG197790.28847592708570036TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATG189370.27619539062752957TruSeq Adapter, Index 8 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA186340.27177614769780783TruSeq Adapter, Index 8 (100% over 39bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG169020.24651499669358953TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG146610.21383010096584523TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC126030.18381425294813092TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTG112770.16447459577053658TruSeq Adapter, Index 8 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATT106390.15516939118584183TruSeq Adapter, Index 8 (97% over 48bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG104010.15169817066678645TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGATG80210.1169859654762325TruSeq Adapter, Index 8 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT71680.10454499445625663TruSeq Adapter, Index 8 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTT70250.10245934515279058TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTG69400.10121962353884223TruSeq Adapter, Index 8 (97% over 45bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG68700.10019867632735534TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1084400.063.2171270
GGATCGG73200.062.833051
ACACTCT181500.062.8316271
AGGGGGG73750.059.0421521
TCCGGCT171750.057.04067256
TGATCGG43750.054.9645271
CACTCTT224950.054.082072
GTCACGC15950.053.7591330
GCTTGTA141800.053.56303460
ACTCTTT227400.053.4836043
TGCTTGT137050.052.9932759
GGTCGGA17000.051.881332
TTCCGGC199200.051.56926355
GCTTCGG76300.050.87315860
CTTGTAA156750.050.843761
TCCCTAC244900.050.5181549
CCCTACA245700.050.3679110
CTTTCCC245100.050.363436
CCTACAC246950.050.14130411
CTACACG248000.050.01368712
Produced by FastQC (version 0.11.5)