FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9843136
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2148042.1822719913653534TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG1221711.241179640309755TruSeq Adapter, Index 5 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG862160.8758997132621148No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237290.24107154467844397No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG141900.14416137296081247TruSeq Adapter, Index 5 (97% over 41bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121800.12374105163232531No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATG101860.10348328012535842TruSeq Adapter, Index 5 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG99950.10154284163095989TruSeq Adapter, Index 5 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG627450.052.6874870
ACACTCT132450.052.0199471
CCTACAC158300.047.22398811
TCCCTAC158800.047.031229
CGACGCT156200.046.8055717
CTACACG159550.046.78820412
CCCTACA160650.046.5331910
ACGACGC159100.046.3703716
GGATCGG47900.046.047051
CACTCTT160050.045.7913252
TCCGGCT51050.045.2502956
TACACGA165650.044.83261513
GACGCTC161550.044.2808818
CACGACG168850.044.2316815
ACACGAC171750.043.93313214
CGGCTTC145400.043.81114258
AGGGGGG39700.043.3881721
TTCCCTA173450.043.2000928
ACGCTCT154950.043.1855719
CTTTCCC175350.042.6933826
Produced by FastQC (version 0.11.5)