FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8191174
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3599554.394425023812215TruSeq Adapter, Index 4 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG767800.9373503724862884No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG224940.274612650152469TruSeq Adapter, Index 4 (100% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224340.273880154419867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC203270.24815734594332878TruSeq Adapter, Index 4 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT198060.24179684133190188TruSeq Adapter, Index 4 (100% over 48bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG120300.14686539438668986TruSeq Adapter, Index 4 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT115460.14095659547703418TruSeq Adapter, Index 4 (100% over 48bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107610.13137310964215873No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATG105470.1287605415292118TruSeq Adapter, Index 4 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA93470.11411062687717292TruSeq Adapter, Index 4 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT138050.055.8404661
AAAAAGG590250.053.35748770
GGATCGG44750.051.3190841
CACTCTT162700.050.702742
TCCCTAC168650.049.990949
CCTACAC168900.049.85478611
CCCTACA170050.049.64111710
CGACGCT165450.049.56141717
CTACACG170350.049.49205812
ACGACGC169100.049.19531616
AGGGGGG36300.048.5097961
GACGCTC166500.048.30298618
CTTTCCC176400.047.835776
CACGACG175450.047.77385715
TACACGA175300.047.73517613
ACTCTTT174100.047.4631583
TTCCCTA177800.047.3395548
ACGCTCT160500.047.05595819
ACACGAC181000.046.57995614
CGCTCTT150700.045.7732320
Produced by FastQC (version 0.11.5)