FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7518512
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG4654386.190560046988021TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1021101.3581144779711731No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG367110.48827480756830605TruSeq Adapter, Index 3 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG315670.41985701426026856TruSeq Adapter, Index 3 (100% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255050.33922935814959126No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC195730.26033076757741425TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATG189830.2524834701334519TruSeq Adapter, Index 3 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC174000.2314287720761768TruSeq Adapter, Index 3 (100% over 39bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG155700.2070888494957513TruSeq Adapter, Index 3 (100% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144980.19283070905519603No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATG111390.14815431564118006TruSeq Adapter, Index 3 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT110800.14736958589678384TruSeq Adapter, Index 3 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATT99360.13215380915798233TruSeq Adapter, Index 3 (97% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT84710.11266857058949963TruSeq Adapter, Index 3 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTG81950.10899763144622233TruSeq Adapter, Index 3 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG72200.058.8119471
AAAAAGG826750.058.69894870
ACACTCT124750.057.3283921
CACTCTT156650.050.4045522
TCCGGCT48800.048.55616456
ACTCTTT163500.048.377783
AGGGGGG39350.048.216591
TGATCGG45200.048.0944671
TCCCTAC168200.048.0033049
CCTACAC169050.047.86545611
CCCTACA169000.047.83819610
CTACACG169800.047.55097612
CGACGCT166600.047.18254517
ACGACGC169200.046.9539516
CTTTCCC173450.046.5304686
TACACGA172300.046.19071213
AAAAAAG1064650.046.1874169
GACGCTC169650.045.5503618
CACGACG176750.045.26509515
TTCCCTA178950.045.2369658
Produced by FastQC (version 0.11.5)