FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11605379
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG4666174.020695920400359TruSeq Adapter, Index 2 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1107350.9541696139350554No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG532880.45916639172232115TruSeq Adapter, Index 2 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300720.25912122301219115No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG258790.22299142492459745TruSeq Adapter, Index 2 (100% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT190980.16456162267514055TruSeq Adapter, Index 2 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC181550.15643608020039673TruSeq Adapter, Index 2 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164640.14186525058768007No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATG148070.127587388572144TruSeq Adapter, Index 2 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG141710.12210717116606015TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATG131610.11340431019098988TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATT127760.11008688298762151TruSeq Adapter, Index 2 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT122310.10539078473869745TruSeq Adapter, Index 2 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT221450.056.640371
AAAAAGG895750.054.95755470
GGATCGG74200.051.6140981
CACTCTT269950.050.1241872
TCCCTAC279850.049.423169
CCCTACA280050.049.3378810
CCTACAC279450.049.3060511
CTACACG280850.049.07272312
CGACGCT277550.048.76112717
ACGACGC282150.048.41269716
TACACGA285750.047.888313
CTTTCCC290150.047.5266156
CACGACG291000.047.26507615
GACGCTC281450.047.19095618
TTCCCTA293750.047.0849088
ACTCTTT289000.046.9408573
ACACGAC295800.046.8528414
ACGCTCT268250.046.55146819
AGGGGGG50150.046.0012251
TCCGGCT72550.045.39577556
Produced by FastQC (version 0.11.5)