FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010259
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG2971273.2976521540612764TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG714850.7933734202313164No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG214590.23816185528074163TruSeq Adapter, Index 22 (97% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160940.1786186168455313No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC142780.15846381330436782TruSeq Adapter, Index 22 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT125740.13955203729437746TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATG124180.13782067751881494TruSeq Adapter, Index 22 (97% over 37bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105660.11726632941406012No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG98780.10963058886542551TruSeq Adapter, Index 22 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT94810.10522450020582093TruSeq Adapter, Index 22 (97% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC93460.10372620809235339TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT152300.056.1311071
AAAAAGG557700.050.5918170
CACTCTT183450.050.3843042
TCCCTAC188650.049.9228139
GGATCGG48950.049.7753561
CCCTACA189650.049.56730710
CCTACAC190250.049.4293811
CTACACG191350.049.1818112
CGACGCT188700.049.0187917
ACGACGC190550.048.94694516
GACGCTC188250.048.0576918
CACGACG195200.047.96023615
TACACGA197150.047.53938313
ACGCTCT180600.047.34161819
ACACGAC200200.047.19972614
CTTTCCC199700.047.1253856
TTCCCTA203350.046.3655668
ACTCTTT202150.045.878783
CGCTCTT174400.045.73357820
TCTTTCC209950.044.2243165
Produced by FastQC (version 0.11.5)