FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238045
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG1619962.596903356740774TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG487860.7820719472206435No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG201300.3226972553099569TruSeq Adapter, Index 7 (97% over 36bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG111560.17883808148225927TruSeq Adapter, Index 7 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105600.16928380606423968No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77090.12358038455958556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC69200.11093219109512677TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT119000.055.2160871
CCTACAC144850.050.64267311
CTACACG145850.050.51141412
CGACGCT143800.050.23364317
CACTCTT143950.050.1091652
TCCCTAC146350.050.1005069
CCCTACA146800.049.9707710
ACGACGC146400.049.8435416
AAAAAGG358900.048.90292470
GACGCTC145100.048.84329618
CACGACG151250.048.4072215
TACACGA151000.048.3714813
ACACGAC153150.048.08090614
CTTTCCC153350.047.813566
ACGCTCT139900.047.73182319
TTCCCTA156300.047.045488
ACTCTTT156900.046.062183
CGCTCTT133550.046.04423520
TCTTTCC161000.044.802575
CTCTTTC163550.044.0398334
Produced by FastQC (version 0.11.5)