FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600885
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG2427653.677764420982944TruSeq Adapter, Index 22 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG585660.8872446649199311No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG247860.3754951040655912TruSeq Adapter, Index 22 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT151720.22984796735589244TruSeq Adapter, Index 22 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC150440.22790883343672855TruSeq Adapter, Index 22 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120970.18326330484472914No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT94120.1425869409935183TruSeq Adapter, Index 22 (97% over 48bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG91910.13923890508621192TruSeq Adapter, Index 22 (97% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86770.13145207044206952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT111450.054.842411
GGATCGG44950.054.048311
AAAAAGG453450.050.3107570
TCCCTAC138800.049.3708159
CCTACAC139350.049.3011611
CTACACG140250.049.0596512
CGACGCT137550.049.05541217
CCCTACA139850.049.05019410
ACGACGC139650.048.74377416
CACTCTT138100.048.6852342
CACGACG143500.047.65551815
TACACGA144100.047.55459613
GACGCTC138650.047.53034218
CTTTCCC146950.046.7524536
ACACGAC148400.046.55400514
TTCCCTA149750.045.9947938
GGGTCGG10000.045.1587681
CGCTCTT125850.044.52240420
ACTCTTT151900.044.445873
TCTTTCC156750.043.1819845
Produced by FastQC (version 0.11.5)