FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10283824
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG1253961.219351867554326TruSeq Adapter, Index 20 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG507450.49344485086481443No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG398330.3873364616119451TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG136160.13240211034338978TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG118930.11564764235560625TruSeq Adapter, Index 20 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG52500.050.209841
ACACTCT119800.048.3608971
AAAAAGG478850.046.0854270
TCCGGCT51550.042.43603556
CCTACAC153000.042.15691411
CTACACG154200.042.03310412
CGACGCT151000.041.76502217
TCCCTAC155550.041.623319
CCCTACA156650.041.33102810
ACGACGC154300.041.25738516
CACTCTT155200.040.884822
CCGGCTT145500.040.7750457
CGGCTTC127550.040.7231358
GACGCTC153100.040.27798518
ACACGAC163650.039.7769814
CACGACG162950.039.5183115
TACACGA163400.039.51656713
ACGCTCT151050.038.46133419
TTCCGGC59950.038.2408155
TTCCCTA173750.037.4448058
Produced by FastQC (version 0.11.5)