FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888180
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG4196103.853812115523439TruSeq Adapter, Index 13 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG808750.7427779481970357No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG270440.2483794353142582TruSeq Adapter, Index 13 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240510.22089091106135278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC231190.21233117013127997TruSeq Adapter, Index 13 (98% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT200580.18421811542424907TruSeq Adapter, Index 13 (97% over 48bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG136130.12502548635309113TruSeq Adapter, Index 13 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT123780.11368291119360628TruSeq Adapter, Index 13 (97% over 48bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA122980.11294816948287043TruSeq Adapter, Index 13 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG716150.051.5500970
ACACTCT141100.049.644341
GGATCGG50850.046.8819661
CGACGCT176250.043.0689617
AGGGGGG42850.043.0535961
CTACACG179250.043.0314912
TCCCTAC179950.042.883559
CCTACAC179400.042.87846411
ACGACGC179100.042.7548816
CCCTACA181550.042.5248910
CACTCTT179250.042.4477162
CACGACG187000.041.1358115
GACGCTC181200.041.0817818
TACACGA187600.040.85500713
ACACGAC193700.040.5079214
AAAAAAG943750.039.6929269
ACGCTCT176300.039.3848619
CTTTCCC198700.038.8554156
TTCCCTA198900.038.762678
GGGTCGG12600.038.3405341
Produced by FastQC (version 0.11.5)