FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11668245
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4070573.488588043874636TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG851750.7299726736968585No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG452300.38763327304149TruSeq Adapter, Index 12 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232810.19952443576561857No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG225720.19344811494787778TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC180870.15501045787091375TruSeq Adapter, Index 12 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT167650.14368056207253105TruSeq Adapter, Index 12 (100% over 48bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA135080.11576719549512374TruSeq Adapter, Index 12 (100% over 39bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127730.10946804767983531No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT119390.10232044322003866TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT190050.052.809361
AAAAAGG767550.052.2058770
GGATCGG54350.049.2090451
CACTCTT234200.046.2226452
CCTACAC241400.045.76975311
CTACACG242700.045.61111512
TCCCTAC242600.045.5724079
CGACGCT238950.045.3602517
ACGACGC240850.045.3511616
CCCTACA243800.045.29067210
AGGGGGG40550.044.7187841
GACGCTC240750.044.16342518
CACGACG250900.043.82750715
ACACGAC253450.043.75938414
TACACGA251600.043.63602413
ACGCTCT231700.042.6106719
CTTTCCC258850.042.5908856
TTCCCTA262550.042.1762128
ACTCTTT258800.042.0311283
CGCTCTT224200.041.0233420
Produced by FastQC (version 0.11.5)