FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053430
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG5467687.751803023493534TruSeq Adapter, Index 11 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG984131.3952502541316778No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG519600.7366628718226451TruSeq Adapter, Index 11 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC345140.4893222162834252TruSeq Adapter, Index 11 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT301440.4273665436532297TruSeq Adapter, Index 11 (100% over 48bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227660.32276495265424054No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG216880.3074816082388285TruSeq Adapter, Index 11 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT185360.2627941299481245TruSeq Adapter, Index 11 (100% over 48bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC176630.25041717292154314TruSeq Adapter, Index 11 (100% over 39bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143220.20305014723333187No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC124690.17677924073819404TruSeq Adapter, Index 11 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT91050.12908613256245544TruSeq Adapter, Index 11 (100% over 48bp)
GGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG77050.10923763332166053TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAGATCTCGTATG72780.10318384105321807TruSeq Adapter, Index 11 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG79100.062.268081
ACACTCT163600.060.4909481
AAAAAGG806200.057.739970
GGGTCGG16000.053.3848081
CACTCTT207100.052.708982
ACTCTTT219000.049.860163
TCCCTAC224750.049.3776559
CCCTACA226800.049.08564810
TGATCGG40250.049.0524981
CCTACAC227400.049.0023111
CTACACG228200.048.8151912
CGACGCT224700.048.53201717
ACGACGC226750.048.47910316
CTTTCCC228900.048.391046
TACACGA230450.047.867813
AGGGGGG35550.047.8568951
GGTCGGA18750.047.7846072
CACGACG232150.047.5775715
TTCCCTA233850.047.4861158
GACGCTC224450.047.33901218
Produced by FastQC (version 0.11.5)