FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_vk4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_vk4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9596689
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173520.1808123614300724No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG100380.1045985756129015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG6600.023.900851
TGTCGGC25150.022.4047330
CGCGAAG14150.021.0575221
GTCGGCG26850.020.59513531
CGGCGGC26600.020.39397433
CCGGGAG5350.020.3121
CGGGCAG10300.020.0798071
CGGGAAG87000.019.823971
CGGCAAG30000.019.1631721
TCGGCGG29300.018.63411732
CGAGCAG18300.018.0061761
CGCGCAT5000.017.527291
GTGGGAC33400.017.080219
CGGCAAA73950.016.6858841
TTAACCG49500.016.54483658
CGGCAAT28350.016.321711
CGCCAAG21150.016.2427831
CGGGGAG11750.016.110191
TAGTCCG31950.016.10268411
AGTCCGC32850.016.08767912
Produced by FastQC (version 0.11.5)