FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_vk4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_vk4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6348695
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116420.18337626866623769No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG71470.11257431645401142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAG7300.024.0078071
TGTCGGC19350.023.5133230
CGGCGGC20800.021.70590833
CGGCAAG19450.021.2651161
CGGGAAG66900.020.9050961
CGCGAAG11400.020.2929151
TCGGCGG23800.019.11692232
GTGGGAC24500.018.42787219
CGGCCTA2155.5106284E-817.9332731
TTAACCG37000.017.68851958
CGCCAAG15300.017.6402471
GTCGGCG25400.017.63712731
CGGCAAA50400.017.3866081
CGCGAAA12600.017.2475151
CGGGCTG4150.016.892241
TGCGGTG12950.016.7813641
CGAGCAG14900.016.7023451
GTGCAGG25000.016.65936714
CTAAACG41500.016.61382721
TAACCGT40600.016.03387559
Produced by FastQC (version 0.11.5)