FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_vk4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_vk4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11586117
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235750.20347628113888372No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG128270.11071008518211925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC44250.026.73340230
CGGCGGC45100.026.3071633
GTCGGCG49350.024.041631
GTGGGAC50750.023.51630619
TCGGCGG50750.023.51630632
CGCGGAG9950.022.198051
CGAGCAG29350.021.8595071
CGGCAAG33850.021.232081
CGGGAAG125550.020.7197531
ATGTCGG59200.019.98231329
AATGTCG61300.019.92579828
CGCGAAG18250.019.7866251
CGGGCAG13450.019.028211
GGCGGCA65200.018.62656234
GAATGTC64600.018.52867727
GCGGCAG66050.017.90996435
CGGCAGT67950.017.46067636
TTAACCG60150.017.3974858
CGGCAAA90650.017.3650461
CGCGAAA23350.017.266661
Produced by FastQC (version 0.11.5)