FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_vk4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_vk4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9458569
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175220.18525000980592307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG6750.021.8141331
TGTCGGC31100.021.04415330
CGGCGGC33800.019.6737533
CGCGAAG13200.019.6539651
GTCGGCG35350.018.71210331
TCGGCGG35200.018.4935632
CCGTATC61300.018.15586355
CGCCGTA60750.018.14740453
GTGGGAC38050.018.1201519
TGCGGTG17650.018.0754491
CGGGAAG80400.018.0524711
CGGCAAG24800.017.9533061
CGGCAAA72050.017.4197311
CGCGAAA17050.017.2721861
CGGCAAT26400.016.9980241
CGTATCA66500.016.94667456
TCGCCGT65100.016.9347952
CGCGGAG7900.016.8635461
CGCCAAG22950.016.8036021
GGTCGCC69500.016.6683850
Produced by FastQC (version 0.11.5)