FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_vk3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_vk3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8769361
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258920.295255264323136No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA101680.11594915524631726No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC87770.10008711010984722No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC50400.038.611155
CCGTATC405650.034.86470855
CGTATCA411550.034.73906756
CGCCGTA429650.034.1716453
CATTAAA425100.034.00224761
GCCGTAT450950.033.8769754
GTATCAT408200.033.7209457
TCGCCGT434350.033.60849452
TCATTAA424200.033.5216160
GTCGCCG451500.033.3861151
GGTCGCC458650.033.26244750
ATCATTA433150.032.60272659
TGGTCGC464300.032.4204849
TATCATT439100.032.0493658
CTCGGTG415900.032.02808844
TCTCGGT420800.031.6218743
ATCTCGG423400.031.5268842
GATCTCG407450.031.52411741
GTGGTCG489800.031.09702148
AGATCTC418000.030.94616540