FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14623854
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3095292.116603461713991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT12200.018.3651652
CCGTATC13000.017.2267955
CGCCGTA12400.017.22025553
CTCGGTG12600.016.96866244
GCCGTAT14500.016.41157554
TCTCGGT13600.015.71977943
GATCTCG12700.015.45152341
CGGGAAG12000.014.5811141
CGTATCA15250.014.4554356
TCGGTGG19000.012.72869245
AGATCTC17100.011.88651740
AGTCCGC7550.011.58133212
AGATCGG31650.011.0439447
GGGTCTC7650.010.98135461
AGCGTCG30600.010.97210717
GATCGGA32550.010.8471938
ACGAGGG8450.010.76364725
GTATCAT21000.010.497107557
GAGTGTA26550.010.41747434
TCGTGTA31700.010.37249321
Produced by FastQC (version 0.11.5)