FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_vk4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_vk4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9596689
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT44650.019.1322121
CGCCGGG4652.5645568E-710.537518523
CTCGCGC19350.010.3131341
CCGGGCT4803.9671795E-710.20822125
GCCGTAA44050.09.77287852
CCCTACA85750.09.7558094
TCGCCCG42950.09.69721737
CCGGTGC43100.09.50105647
TCGCGCT19950.09.4745672
CGGTGCC44000.09.46580548
AGGCCGC7101.364242E-99.36609616
CGACGCT84550.09.35530911
CCTATGC26600.09.34200412
GCCCGCA45100.09.31253739
ACGACGC86900.09.18286910
GACGCTC83250.09.16506512
GGTGCCG45250.09.12697249
GTGCCGT45650.09.12366950
GGCGCGT20750.09.10833546
CGCCCGC47400.09.00834138
Produced by FastQC (version 0.11.5)