FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_vk4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_vk4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6348695
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT30850.013.9570231
CGGGCTG3052.939234E-612.62283726
CCCTACA59000.012.2212874
CCTACAC62350.011.7330685
ACGACGC59100.011.25201410
CGACGCT59250.011.22352711
GACGCTC58650.011.09964512
CTACACG64900.011.0563296
TACACGA68950.010.3552367
ACGCTCT64600.010.29402513
TCCCTAC72400.010.1044553
TCCGATC55300.010.06320120
TTCCCTA79150.09.8176442
CCGATCT54350.09.78832121
ACACGAC73700.09.5453698
CCGGGCT3350.00122424759.40290125
CCGCCGG4905.6736335E-69.2856322
CGCTCTT72300.09.24611414
CACGACG76300.09.22019
CGCCGGG3450.00160110859.13035223
Produced by FastQC (version 0.11.5)