FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_vk4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_vk4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11586117
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT62900.015.6958041
CGGGCTG6450.011.93780226
CGCCGGG6900.011.15924923
CCGGGCT7101.8189894E-1210.84490425
CCCTACA117400.010.1673474
GACGCTC110600.010.06313712
CGACGCT112050.010.05785711
CCTACAC121650.010.0423355
ACGACGC114250.09.95608410
CTACACG125800.09.6553996
ACGCTCT119700.09.444313
TCCGATC106750.09.08182920
GCCGGGC8901.2732926E-119.04480624
TACACGA133500.08.9924117
CCGCCGG9403.6379788E-128.93603322
CCGATCT107750.08.6402421
GGCCGCC9404.0017767E-118.56369917
TTCCCTA148950.08.5074012
CGCTCTT132900.08.4272614
TCCCTAC146600.08.3572423
Produced by FastQC (version 0.11.5)