FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_vk4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_vk4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9458569
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT64550.018.00110656
TGCCGTC74650.016.50327955
CTGCTTG65950.016.3456664
TATGCCG79400.015.78046953
CCGTCTT76000.015.61162357
ATGCCGT79550.015.53073154
CGCCGGG5550.015.13476223
TGCTTGA75500.015.01981665
GCTTGAA78200.014.94879866
GGCCGCG3806.730261E-1114.73647953
GTATGCC87350.014.58464352
CGGGCTG5850.014.3586226
CCGGGCT5650.014.247437525
CTCGTAT87450.013.44735349
CGTATGC96200.013.20653451
GATCTCG82500.012.939074546
TCGTATG101600.012.57350950
CGCTTGC15300.012.12388423
ATCTCGT89200.011.96719547
TCTCGTA94150.011.89561848
Produced by FastQC (version 0.11.5)