FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_vk3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_vk3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8769361
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA78550.037.7398466
GAGATCG185500.036.8918236
TGCGTGA59700.034.70645565
CTGCGTG57650.034.36212564
CGTGAAA91850.033.83737667
CGAGATC99100.033.627195
CTGCTTG153900.033.47588364
GCTTGAA190750.033.35740366
TGCCGTC241000.032.98074755
TGCTTGA169200.032.53803365
GTGCGTG18350.032.42468364
ATGCCGT251300.032.408954
GCCGTCT191950.032.3646356
TATGCCG287650.032.35298553
GGCGTGA23500.032.1698365
GCGAGAT34800.032.0877084
CGCGAGA15300.032.0312423
CCGAGAT34900.031.9957684
GTATGCC305850.031.6636652
CCGTCTT213150.031.59221857
Produced by FastQC (version 0.11.5)