FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14623854
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1676011.1460795492077533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11150.019.4373661
GAGATCG13550.016.5074356
CACACGT35300.013.37751419
GTCACCT23350.013.19395536
GCACACG37750.012.78601918
AGTCACC24950.012.62921935
AGATCGG42400.012.6075967
ACGTCTG33650.012.57345622
CAGTCAC30150.012.42457934
GATCGGA43050.012.4172398
ACACGTC36450.012.37169420
AGCACAC40800.012.0852717
GAACTCC37850.012.02405128
AGAGCAC42400.011.95892415
CACGTCT36800.011.87584521
CGTCTGA36500.011.59403223
TGAACTC40050.011.44751327
TCGGAAG46050.011.38068410
CCTTGTA21250.011.35987240
CCAGTCA33700.011.21975933
Produced by FastQC (version 0.11.5)