FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_ds1352-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_ds1352-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12771792
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG37700.028.8813441
GCACACG14750.018.0335911
AGTCACG17000.015.64679128
ACACGTC20650.013.38960213
GTCACGT20250.013.30841429
CACACGT20050.013.26660612
ACGTCTG22650.012.51634215
TCACGTG22250.012.42675430
GATCGGA21150.012.4150721
CGTCTGA24650.012.3527316
TGCCGTC22750.011.99979550
CACGTCT24950.011.64308714
GCCGTCT21400.011.61195151
CACGTGA23400.010.91861631
AGCACAC26250.010.26649110
CGATCTC20850.010.23963340
GATCTCG22800.09.82439341
CGTATGC28800.09.60053146
GTATGCC28400.09.48927447
CAGTCAC28700.09.39008327
Produced by FastQC (version 0.11.5)