FastQCFastQC Report
Sat 28 Sep 2019
HTFH3AFXY_n01_ds1351-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH3AFXY_n01_ds1351-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11499009
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27250.017.9874291
GTCACGC10250.09.90226129
GCACACG11950.09.37221611
TCACGCC13050.08.31402730
AGTCACG13800.07.60855828
ACACGTC16750.06.89539813
CACACGT17150.06.734572412
CACGCCA15805.456968E-126.645449631
ACGTCTG18900.06.48136515
TGGGGGG23500.06.10834461
CACGTCT20755.456968E-125.734836614
GATCGGA18201.2241799E-95.5787151
CGTCTGA20102.6502676E-95.22378716
Produced by FastQC (version 0.11.5)