FastQCFastQC Report
Thu 5 Oct 2017
HTFGGAFXX_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFGGAFXX_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83446258
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT909374110.897721740859847No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGCATCCGCCGTGGGCGGT68626458.224029650316975No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC62424997.4808615144851665No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA16174561.9383205895224205No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGACGGC11136031.334515203785411No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAAACCACACCGTGGTAATC10986521.3165982829331904No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCTACCTTAGACGGC9631361.154199149349513No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGCCCTGCCTTAGACGGC8814071.0562570702691065No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAA8092320.9697642763082319No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTCGGCCGC7706720.9235548944567412No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGTCCCGCCTTAGGCGGC7646320.9163167029011654No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA6061160.7263549193542028No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG6055260.7256478774638402No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC5481450.6568838593097848No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAGACCACACCGTGGTAATC5447710.6528405383977793No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC3893390.46657454669806764No Hit
GGAAACCGTGTTACGACTTTTGCATCCTTTAGAGGGCTCTATTTACGCAA3628210.434796009666485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3028600.36294018121220006No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGGAAGG2741380.32852042328848347No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATTAGGTTTGACTAG2069920.2480542626608853No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA2009240.2407825165749194No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTCGGTCAA1867910.22384586736052323No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTTGAACAC1745050.20912261877578742No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG1685520.20198868593963795No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1613440.1933507911163614No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCCAGGCGCGGGGGCTCGCGGCC1503240.1801446866556916No Hit
AGATACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTGAACTG1406870.16859593632107506No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAGATGACACGATTCGAGGCA1197560.14351272647840002No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC1195450.143259869124389No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGAACAC1181130.14154379457015318No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCAT1045530.1252938148526684No Hit
TGATACCTTGTTACGACTTGTGCTTCCTCTAAATGATACAGTTTGAAGCC978580.11727068696118165No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA947890.11359287075521109No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAACGCG859520.10300282128888272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGTT43317500.093.94636
TGTTACG43741700.093.9396449
CTTGTTA43263500.093.934897
ACCTTGT43246350.093.927455
GTTACCT17288450.093.698522
AAACCTT17027800.093.66743
TTGTTAC43361300.093.387698
ACCGTGT601350.093.073245
CCTGAGG1660500.093.0598794
CTCATGG2991550.092.8756994
TACCTTG26481200.092.8613364
CCGTGTT607250.092.6354146
GGCTACC7641300.092.4318541
GATACCT1418000.092.04082
GCCTTGT168950.091.8859945
AACCTTG17506300.091.51014
GAAACCT17154900.091.508312
CGTGTTA617000.090.849257
CCGGAAG476600.090.3506894
GGAAACC17614950.090.335181
Produced by FastQC (version 0.11.2)