FastQCFastQC Report
Thu 5 Oct 2017
HTFGGAFXX_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFGGAFXX_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83446258
Sequences flagged as poor quality0
Sequence length12
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG3773603745.2219642970689No Hit
TCGATGAACTCG7348680.8806482370965035No Hit
CATCAAACCTCA7324240.8777194059438831No Hit
TTCTACGGAGCA6537040.7833832405043255No Hit
GCTGATGAGCTG6326770.7581849865574559No Hit
GCCTCCCTTATG6150280.7370348470269332No Hit
TGCTCAGTATGT6133600.7350359557165522No Hit
ACAGGAACTCGC6053160.7253962184859146No Hit
GTATGCGCTGTA6013650.7206614345726563No Hit
ATTCCTGTGAGT5958510.71405358883798No Hit
GATTGCCGCAAG5822040.6976993504010689No Hit
CCTTTCTTCTCA5736750.6874784007690314No Hit
GTCCGGCTTCTT5685980.6813942453836576No Hit
GATAGTGCCACT5641220.6760303140255852No Hit
CTTGCCTTGTGC5525950.6622166328896377No Hit
GGTAATCCTGTG5353920.6416009690931858No Hit
GCACACACGTTA5338920.6398034049651453No Hit
TCTATCGGGTTA5297840.6348804760064856No Hit
CCACTAATTGAC5232440.6270430964082296No Hit
AGCTGTTGTTTG5192930.6223083124949713No Hit
GCTTACATCGAG5189150.6218553263347052No Hit
ACTCTTCTAGAG5183340.6211590698291108No Hit
CCTTATAGGCGA5171220.6197066380136542No Hit
CTATTTGCGACA5100730.6112592849879499No Hit
ACCACCAGAACG5087030.6096175097510065No Hit
CCTGTATCCCGT5068060.6073441903170781No Hit
GGTGTCTATTGT5052820.6055178651629891No Hit
CGATCCGTATTA5018650.6014230140793132No Hit
ACCTAGAGTCGG5001950.5994217260167616No Hit
GGATCGCAGATC4988090.5977607767624523No Hit
TACACGATCTAC4829940.5788084589724802No Hit
TCGAGACGCTTA4783970.5732995241080793No Hit
CGAGGCTCAGTA4741940.5682627494213102No Hit
AGGAGTAACACA4734280.5673447933399243No Hit
TCACAGATCCGA4731550.5670176366686209No Hit
CATAGTTTAGCC4636970.5556833956532838No Hit
GCGCTAAAGTTC4634170.5553478503493829No Hit
GACAAGGCTCCA4607180.5521134332949957No Hit
TCGTTTCGGGAA4552810.5455978625188921No Hit
TGAACTGCCGTA4513370.5408714672382313No Hit
CTTGATGCGTAT4475070.5362816868313016No Hit
ATTGCAGGTACC4470170.5356944825494752No Hit
TCCGAATTCACA4380180.5249102961573184No Hit
GCCTTCTAAGGA4373660.5241289549496635No Hit
ATAAGGCCTTCT4360030.5224955683453175No Hit
GAGTCAACCGCA4353170.5216734823507604No Hit
TTCAACAGGTGC4325740.5183863367486173No Hit
TAATGACCACGC4235860.5076153324933995No Hit
GTGTACCTATCA4199970.5033143607230417No Hit
GTCAAGAACCTC4170400.4997707626386314No Hit
ACCGTGCATAGG4168720.49956943545629096No Hit
GGAAACCACCAC4160920.49863470210970995No Hit
GCAATAGCTGCT4156860.4981481614190537No Hit
TAGGATTGCTCG4086940.48976911583021493No Hit
GAGGCTCATCAT4049930.4853339259382967No Hit
TTACTGTGCGAT4045280.48477668105860416No Hit
TTGAAGTCCATC4044930.4847347378956166No Hit
AGATTCGTTGTC3955700.4740416280859473No Hit
GACAATCTGCTT3854500.4619140621021017No Hit
CTCAGTATGCAG3847070.46102366867067907No Hit
TCGACCAGCAAT3821640.4579761982856079No Hit
TTAGGGCTCGTA3820880.45788512170312057No Hit
AAGTGTTTGCCG3732050.4472399469368656No Hit
CTACTGATATCG3705120.4440127201389905No Hit
GGCTCTGCTCTT3642590.436519274477233No Hit
ATGACTCATTCG3621010.4339331788850256No Hit
TGCTCGTAGGAT3587600.4299294043838371No Hit
ACAAAGGCCCTG3460320.41467647356937204No Hit
CTAGAGACTCTT3363830.4031133427217311No Hit
ATGGCAGCTCTA3264130.39116553315068964No Hit
GACAGCGTTGAC3246060.38900006756444366No Hit
TGCCAGGCTAGA2950190.35354371432689047No Hit
CGATGTCGTCAA2827170.3388012917247889No Hit
GGAGACAAGGGA2786300.33390352866392164No Hit
CACGCCATAATG2760540.3308165118680337No Hit
AATGCCTCAACT2684760.32173521789317383No Hit
AGTTTCTCTAGG2664340.3192881339268682No Hit
TCTCACTAGGTA2579490.30911991284258666No Hit
TAGAGTACCGGC2556160.30632410143544125No Hit
TACAGATGGCTC2460210.2948256828964098No Hit
AGTAGAGGGATG2453560.29402876279964524No Hit
AGGATCCGCATG2256620.27042794417456084No Hit
TCTAGCGTAGTG2242640.26875261440722725No Hit
CCTCGTTCGACT2141490.25663104030380846No Hit
AGAGGAGACCAA2129710.25521935327525413No Hit
CGGAGCTATGGT1983000.23763797772693415No Hit
CCAGTGTATGCA1938290.23228003824928853No Hit
AATTTGATCCCG1915920.2295992709463377No Hit
ATTTCGACATGC1848810.22155696903748517No Hit
TAGGAACTGGCC1817860.21784799505329527No Hit
CGCAGCGGTATA1801410.2158766663928777No Hit
NNNNNNNNNNNN1694210.203030074757816No Hit
CTAGCGAACATC1633070.19570320337192354No Hit
AGAGTCCTGAGC1627240.19500455011415851No Hit
TGAGTCACTGGT1619710.19410217292188225No Hit
ACTGTCGAAGCT1604950.19233336981989055No Hit
GGAGTCGGTCTA1600340.19178091844453948No Hit
AAGGAGCGCCTT1598810.19159756690347937No Hit
ACGTTAGCACAC1595500.19120090441922513No Hit
CAGGCGTATTGG1564720.18751230282848633No Hit
TCCTCTGTCGAC1514820.18153240616253877No Hit
GATCTATCCGAG1480830.17745912584839935No Hit
CGACTCCAAGAT1436850.17218866782498504No Hit
TTGCCACTAGAT1397090.16742392450959273No Hit
TCGAGGACTGCA1373700.16462092284593519No Hit
CGTGTTTCAAAC1356450.16255372409868876No Hit
ACGTGTACCCAA1339060.1604697480862473No Hit
GCACCCAAAGTG1230540.14746497080791807No Hit
CCTACGCCTCTA1212820.14534144838465973No Hit
ACCTGTCTCTCT1168660.14004941959170897No Hit
TAGCTCGTAACT1133980.13589345132767966No Hit
ATGGGCTCTGAT1131990.13565497448669298No Hit
TGGCTCTACAGA1092970.13097891100161735No Hit
TCCCATTGAAGG1010840.12113664821255377No Hit
CTGCATAGATCT1006470.12061295786325134No Hit
CTAACCTCCGCT960730.11513158564881362No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCA2150.06.0005051
GACAGAG909.142854E-66.0005051
TCGCATT650.00126091046.0005051
AATTTGG1202.5662303E-86.0005051
ACGTTTC600.00339386826.0005051
TAGAATT953.4261338E-66.0005051
TTCACGG2800.06.0005051
TTCACAG550.00915387556.0005051
GCTTGCC1900.06.0005051
CTTGGCT600.00339386826.0005051
ACGTGTC550.00915387556.0005051
ACGTGTA135900.06.0005051
CTTGAAG600.00339386826.0005051
ACCGTGA1303.6416168E-96.0005051
ACTTTCT1101.8132232E-76.0005051
AAGGCAG704.6932301E-46.0005051
TCGTTAC751.7497387E-46.00050451
TAGAAGT1156.8190275E-86.00050451
TTCAACG1552.7284841E-116.00050451
CTTGACT852.4425119E-56.00050451
Produced by FastQC (version 0.11.2)