FastQCFastQC Report
Thu 5 Oct 2017
HTFGGAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFGGAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83446258
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG61870617.414425941064967No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC59091407.081372061045565No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG56856716.813572155626199No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTCCTCGGATCGGCCCCGGCGG30992833.714106628963518No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG14767031.7696455603797117No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTGGAG9236031.106823747566967No Hit
ACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGG8653691.0370375146120991No Hit
ACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGA7474710.8957513709002985No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGAATGCCAAACTGGCTACC6801480.8150730977055916No Hit
ACACCATGGGAGTCTGCAATGCCCAAAGCCGGTGGCCTAACCTTCGGGAA5654730.6776493201169069No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTGAGCTAACCGCAAGGAG5445950.652629624206756No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAAGA5337240.6396020777828049No Hit
ACACCATGGGAGCGGATTGCACCAGAAGTAGTTAGCTTAACCGAAAGGAG5302960.6354940445621899No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCCGCAAGGG4482940.5372248088104802No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCAGCTTAGAGAA4226510.5064948508535877No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATTGCGGCGACGTG3983150.4773311704402611No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATGCGCTTAGCCTG3285110.39367972617777536No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGAAAGGG3212190.3849411677633286No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG3077980.3688577623217089No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAAGG2811020.33686591434693214No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATATAAATAATT2767690.33167335076906623No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGTGAGCCAACTAGCAATAG2697860.32330509056499573No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGGCCCCGCCGG2510330.3008319438362353No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG2383760.28566409772383083No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGTAAGGAG2285770.2739212104633859No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTAAGCTAACCGTAAGGAG2128280.2550479854950476No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2032590.24358072473423553No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCTTAGGAGG1969790.23605492291817326No Hit
GCTACTACCGATTGAATGGTTTGGTGAGGCTTCCGGATTGACCTAAGAAC1952670.23400330306003655No Hit
GCTACTACCGATTGAATTATTTAGTGAGGTCTCCGGACGTGATCACTGTA1853870.22216334733667745No Hit
ACACCATGGAAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTTATGT1702360.20400675126738457No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTTGCCTCTGATTAGGGTGTTCA1683310.20172384482477335No Hit
GCTGCAACCGATCGGAGGGTCCTGTGAATTCATCGGATTGGCCTACTTCG1680980.2014446231968844No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAGAGAA1494760.17912846373530614No Hit
GCTGTCCGGGACTGAGCTGTCTCGAGAGGACTGCGGACTGCTGTATCGAG1435100.17197895201004698No Hit
GCTCCTACCGACTGGGCGCGGCGGCGAGCGTCCCGGACCCGCGAAGGGCC1386800.16619079551775706No Hit
GCACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC1356200.16252376469655477No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGAGTAACCATTTGGAG1306930.15661936572398488No Hit
GCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1259100.1508875329077069No Hit
GCTGTTACCGATTGGATGGCTCAATGAGTAGCAAGGAGGTTTGTTGGGTC1208400.14481176615493052No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTCACCTCTAATTAGGGTGTTCG1135190.13603845483400825No Hit
ACACCATGGGAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTATGGA1077400.12911303943671146No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACTGGCTAGCTTGG959670.11500455778376546No Hit
GTTGTTACCGATTGAATGGTTTAGTGAGTATTCGGGAGGGGGTATCTGCG952160.1141045773436599No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGCGAGCCAACCAGCAATGG935690.11213085193107161No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG922740.11057895490053012No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA895270.10728701579404555No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCTCA861130.10319575983862572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATGG19690200.093.8776863
CTAGTAC1672200.093.8774642
CCATGGG19509250.093.8773354
CTACTAC11835150.093.871032
CCGATTG17020850.093.856478
TGGGAGT17517350.093.832477
ATGGGAG19519050.093.828286
TACTACC11812050.093.818353
ACTACCG11821100.093.797794
ATGAGAG4451150.093.69686
GGGAGTG7554850.093.689928
GGGAGTT9181450.093.680838
CCATGAG4447800.093.67554
CTCCTAC2966050.093.672852
CATGGGA19532850.093.6691745
CGATTGA6405850.093.5856259
CATGAGA4444650.093.581255
GCTAGTA1669000.093.5408551
GAGAGTT2659050.093.439928
GGAGTTG8242900.093.436149
Produced by FastQC (version 0.11.2)