FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB11LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB11LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158876
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGTGTAGATCT69894.3990281729147265Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44852.8229562677811626No Hit
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGT8390.5280847956897203Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGT6460.406606410030464Illumina Single End PCR Primer 1 (97% over 34bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGG4070.2561746267529394Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAG2890.18190286764520758Illumina Single End PCR Primer 1 (97% over 34bp)
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGT2870.18064402427050028Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAG2180.13721392784309777Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGTG1950.1227372290339636Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAGAGTGTAGATCTC1900.11959012059719529Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)