FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB10LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB10LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34735
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGTGTAGATCT24967.185835612494603Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17044.905714696991507No Hit
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT1320.38002015258384914Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT1230.3541096876349503Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCG690.19864689794155752Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGG650.18713113574204693Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGTG480.13818914639412697Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGAT400.1151576219951058Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG390.11227868144522817No Hit
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCGATGGT370.10652080034547287Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)