FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB09LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB09LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12143
Sequences flagged as poor quality0
Sequence length151
%GC74

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196916.215103351725276No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATCT196116.149221773861484Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGG2692.2152680556699336Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGT2311.9023305608169314Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGT1901.5646874742650085Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGT1351.1117516264514535Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAG970.7988141315984517Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGC800.6588157786378983Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG690.5682286090751874No Hit
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAG530.43646545334760767Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTA500.4117598616486865Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATC480.395289467182739Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTG430.3541134810178704Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATC320.26352631145515937Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG270.2223503252902907No Hit
CCAGACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTG270.2223503252902907Illumina Single End PCR Primer 1 (96% over 33bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAG270.2223503252902907Illumina Single End PCR Primer 1 (96% over 33bp)
AGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGG260.21411512805731697Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTG260.21411512805731697Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCAGGGTGTAGATCT250.20587993082434325Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGG240.1976447335913695No Hit
AGATCGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATCTC200.16470394465947458Illumina Single End PCR Primer 1 (96% over 28bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATAT180.14823355019352713Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGGGGGGGGGGGGGGG170.1399983529605534No Hit
CCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTA170.1399983529605534Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGGG170.1399983529605534No Hit
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGG160.13176315572757968Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGTGGG160.13176315572757968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG150.12352795849460595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG140.11529276126163222No Hit
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGC140.11529276126163222Illumina Single End PCR Primer 1 (96% over 33bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAGGGTGTAGATC130.10705756402865849Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGGGG130.10705756402865849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)