FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB07LB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB07LB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25418.54014598540146No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAGATCT15311.167883211678832Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGT322.335766423357664Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGG211.532846715328467Illumina Single End PCR Primer 1 (97% over 34bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGT191.3868613138686132Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAG80.583941605839416Illumina Single End PCR Primer 1 (97% over 34bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACT80.583941605839416Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTA70.5109489051094891Illumina Single End PCR Primer 1 (97% over 34bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCC60.43795620437956206Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGT50.36496350364963503Illumina Single End PCR Primer 1 (97% over 34bp)
GTGACTGGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGT50.36496350364963503Illumina Single End PCR Primer 1 (97% over 34bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAG50.36496350364963503Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG40.291970802919708No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAGATC30.21897810218978103Illumina Single End PCR Primer 1 (97% over 34bp)
AGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGG30.21897810218978103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG30.21897810218978103No Hit
CCCATCCTGCTCCGCGGGCTTGGGTTGGTGTCTCCACAGAGGCACCAACC20.145985401459854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGGGGGGGGGGGGGGGG20.145985401459854No Hit
AGATCGGAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAGATCTC20.145985401459854Illumina Single End PCR Primer 1 (96% over 26bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTG20.145985401459854Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGGG20.145985401459854No Hit
CTCTTCCGAGAGCTCAGCTAGTCTTCTTCAGATCGGAAGAGCGTCGTGTA20.145985401459854Illumina Single End PCR Primer 1 (100% over 21bp)
CAGACGTGTGCTCTTCCGATCTGAGATCGGAAGAGCGTCGTGTAGGGAAA20.145985401459854Illumina Single End PCR Primer 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGGGGG20.145985401459854No Hit
CCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAGAT20.145985401459854Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTACGGTGTAGATCT20.145985401459854Illumina Single End PCR Primer 1 (97% over 34bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACTCCGGTGTAGATC20.145985401459854Illumina Single End PCR Primer 1 (97% over 34bp)
CTGCGCAATCCAAACATGGAGAAGCTTCAGAAGGGGAGATCGGAAGAGCG20.145985401459854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)