FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB06LB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB06LB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length151
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCT129.090909090909092Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43.0303030303030303No Hit
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCGTCGTGTAGGG21.5151515151515151Illumina Single End PCR Primer 1 (100% over 24bp)
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGT21.5151515151515151Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTTGGGGGGGGGGGTGGGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGTTGGTGGG10.7575757575757576No Hit
CACCGCCCCGCCGTTCTTGGACAGCAGCGCACCCTTGGCAAAAATCGCAG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTAAAGGGTGGGGGGGGAGTGGGTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGCGGGGAAAGTAAAGAATATCTTAAATATTTAAAAG10.7575757575757576No Hit
GGGTCCATCATTTAATCAATTAAAAATATAGATTTAAAAAATATAATAAT10.7575757575757576No Hit
AGCAAATTTACTTACTCAAGCAGCCATAGAAGAAAATCAGAAAGATCGGA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTTGGTGGGGGGGGGGGGTGTGGTGTG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGGGGGGTGTGTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTAGGGTGGTGGGGGGGGTTTGTTAGG10.7575757575757576No Hit
ACGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGT10.7575757575757576Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGAAGTTGGGGGGAGGGTTGGTGGG10.7575757575757576No Hit
TGAGCAAATCCCATGGGGTGGATACACTAAGACTAACCCTAGGATATCCC10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGGGTTTTTGG10.7575757575757576No Hit
GGGGGGGGGTGAAGAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGGGGTGGGGGGGGGTGTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTTGGGGGGGGGGGGGGTGAGGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGAGAGGGGGAGGTAGGGGGTTGGG10.7575757575757576No Hit
CGATGTGGTGGCCGTCTCCTTGATTGTCGTGGTCGGCGGCGTGGATGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGAGGTTTGGGGGGGGGGGGTGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGCCCGCGGGGGGGGGGGGGGGGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGGGGGTGGTGGTGGG10.7575757575757576No Hit
TTGGGGTGGTTGTGGTCGGGGGTGGTTGGGGGTGTTTAGGGGGGTCGGTT10.7575757575757576No Hit
GGGGGGGTAAAAAAAAAATAAAATAAAAAAATAAAAAAAAAAATAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGTTGGGAAAAAGTTGGTGGCAGGGTTTTTATA10.7575757575757576No Hit
GGGGCGCAGAACATACAGTAAACAAAAAAAAATAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGATGTGGGTGGGGGAGGGGAGAGGTG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGTGGTTTGGTTGGGGGGGAGGGTTTTTTGT10.7575757575757576No Hit
GCCTGCTTTTGCCCTTCCCCTGCTCGGCAACTGCACACATCGTCTCGTCC10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGTGGGGG10.7575757575757576No Hit
GGGGGGGGAAAAAGACGATATGGATATAAACATGAAAAAAAATAAATAAA10.7575757575757576No Hit
GGGGGGGGGGGGCGCGGGGATTAAAAAAAAGAAGCTGGGAAAAAAAAAAA10.7575757575757576No Hit
GGGGACAGAAAAAAAAAAAAAAATAAAAAAATAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTTGGGGGGGGGGTTGGTGGG10.7575757575757576No Hit
GGGGGGGAAAAAAAAAAAAATATTAAAAAATTAAAATAAAAATAAAAAAA10.7575757575757576No Hit
ATGTGCTATGAAAGCAGTGAATAACCGCATACTTGTAAAGGAGATCGGAA10.7575757575757576No Hit
GGGGGGGGGGAACAAAATAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGTGTTGGGTGGGGGGGTTGTGGTG10.7575757575757576No Hit
GGGGGGGGTCTAACAAAATATAATAAAAAATATTAAGCAAAATAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGTGGGGGGGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGTGGT10.7575757575757576No Hit
GCGTCAGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGTGAGGGAAAAAAATAAAAAAAAAAATATAAAAAAAGATAAAAA10.7575757575757576No Hit
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGACTGTGCTCGAAG10.7575757575757576Illumina Single End PCR Primer 1 (96% over 33bp)
TCTCGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGTAAG10.7575757575757576Illumina Single End PCR Primer 1 (96% over 28bp)
GGGGGGGGGCCCGCGGGGGCTTTTAATAAAAATTTATAAAATTATAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGTGTGTTGTTGTG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTGGTTTTTTGGGGGGGGTTGTGTG10.7575757575757576No Hit
GGGGGGGTTATTGACATTGTATTTAAAAATATTGTTAAAATTTTTTATTA10.7575757575757576No Hit
GGGGGGGTTTAAAAAAATTTAAATAAAAAAAATAAAAAAAAAAAATAAAA10.7575757575757576No Hit
GGGGGGGGTGAAGACCATCAGAAGAAAAAAATTGATAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGTGGTGGTTTGGGGGTTTGGTTTGTTTT10.7575757575757576No Hit
GGGGGGGGGGGGGGCCGGTGGTGTAAAGAATTAGTATAATTAAAGATAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGATGGTGGGGGGGGGGTGGGGGGGGG10.7575757575757576No Hit
GGGGGGGGGAGGGCCGCCATATCAAAAAAAAAGAAATAAAAATAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGTGGTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGGGG10.7575757575757576No Hit
GGGGGGGGCCAAACAAACTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGCCAACGCGAAAAGGCAGAAAAAAAGGTAGAAAAAATAAAAAA10.7575757575757576No Hit
GGGGGGCGAAAGCAAAAAATTTGCAAAAAAATTTTGGAAAGAATATAATA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGGGGGGGGTGGTGGG10.7575757575757576No Hit
GGGGGGGGGAAAAAAAAATAATTAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
AAATATGATCCCGATGCCATAATAATAATAAGATAAGCAATAGCCTGTAT10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTTGTGTGTGGGGGGTGGGTGGGTGGG10.7575757575757576No Hit
GGGGGGCACTACAAAAAGAATTAAAAAAAAATAATAAAAAAATATAAAAA10.7575757575757576No Hit
GGGGGGGGGTTAAAAAAAATAAAAAAAAAAATAAAATAAAAAAAAAAAAA10.7575757575757576No Hit
GCAAAGGACCTTGTGAAGATGCTGGAGGAACAAGTACAAAAGTATCTATA10.7575757575757576No Hit
GGAATACTTCCGCCGTTCGGGCCTTGTTGGCGGCCAAACCGGACTGTCTG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGTTGTTTATATTTGTGTGGGTTTTTTTTTTAA10.7575757575757576No Hit
TGGTAAGAACAGTATTTGGATTTGCAACAATGTGAGAAAAGCAGAGTGTT10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGTTGTGGA10.7575757575757576No Hit
TCCCCTTCGGGGCTGGCTCCGACTCAGCGAAGGCCCTCTGGTGCCGGCGC10.7575757575757576No Hit
GGGGGGGGGGCGGGCCGCGGTGGTAAAGGGATTGTTAAAGGATTCTATTA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGTGGTGGGGGGGGTGTTGTG10.7575757575757576No Hit
GGGGGGGGGGGGCTGCTCAAAGAGAAAAAAAATCATAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGCACGCAAAAAAAATAAAAAAAATAGAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGAGGGTGGGGGAGGGGGTTGGGG10.7575757575757576No Hit
GGGGGGGTCAGATAAAATTTTTCGAAAAAAAATAGAAAAAAATATAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGATAGTGGGGGGGGGGTGGTGGTGTG10.7575757575757576No Hit
TACGCTCTGCCGCTTACGCTCTGCCGCTTACGCTCTGACGCTTACGCTCT10.7575757575757576No Hit
GGGGGGGTGTAAAAAGATTTATAAAAAAATATAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGTATAAGAAAAAAATTATAAAAAAAATTAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGTTTAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATC10.7575757575757576Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGTGTGTGGGAGGGGGTGGGAGGGTGTTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTTGGGGGGGGGGTTGTGGG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGAAAAAAAAGAA10.7575757575757576No Hit
GGGGGGGTGTAAAAACTAATAAAAAAAAAAAAATAAAAAAAAAAAAAAAA10.7575757575757576No Hit
TTTCACGAGTTGAAAAAACTGAAGGTGTACTACCGCAAAGGTGTCTTTTT10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGTTGGGAATAATTTTTGTTGAATTGTTATAAG10.7575757575757576No Hit
GGGGGGGGGGGCGGGGGGTGGTATAATAATATTTATGATATGATATAAGA10.7575757575757576No Hit
AGCCGTCACAATCTACCAGAAGGACATTGAAGATGAATTTGAAATTCAGG10.7575757575757576No Hit
GGGGGGGTAATCAGAACATAAAGTTAAAAAAAAAGAAAAAAATAAAAAAA10.7575757575757576No Hit
GGGGGGGTTTAAAAAAATTATTTTAAAAAATATAAATAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGTTGGGGGGGGGGGGGGGTTGTTTGG10.7575757575757576No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT10.7575757575757576Illumina Single End PCR Primer 1 (100% over 31bp)
CTGAACTGTTCTTTGTATTATTTAAGAATTGAGCTCTGAAAAATCCGAGA10.7575757575757576No Hit
GGGGGGGGGGGGCGGGGGGGTGTAGAGATTGTTGTGGGAAAATATAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGTGGTTGGTGGGGGGGTTGGGTTGGG10.7575757575757576No Hit
GGGGGGGCAATACTAAAATATTTAAAAAAAAATAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTGGGGGGGGGGGGTGGGTT10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGGGGGGGGGGGTGTGTTGGG10.7575757575757576No Hit
GGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG10.7575757575757576Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGTGGTTTGGGG10.7575757575757576No Hit
GCGCACAAAAAAATAAAAAATTAAAAAAAAAATATAAAAAAAAAAAAAAA10.7575757575757576No Hit
GGGGGGGTGAACAAAATTTATATAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GCATATGCTAGTACATGCTACCAAATATTCCCAAAAAGTGTACCGCGACC10.7575757575757576No Hit
GGGGGGGTGGTCCTGTTAATATTTATAAAAAAAATAGAAAAATAAAAAAA10.7575757575757576No Hit
GGGGGGGGGTGGGGTGCAGATTTATAATAATTAAAATAAAAAGGAAAAAG10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGTTTTGGTGGGGGGAGTGTTTTTTA10.7575757575757576No Hit
GTTTCTTTATGAAAGTTGCTTAAAAAAATCAAATAATCCATTTTTGAAAA10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGTGGGGATAAAATTTTGTGGTTTGTTTTAAAG10.7575757575757576No Hit

[FAIL]Adapter Content

Adapter graph