FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n02_HRB05EB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n02_HRB05EB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5222
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGTGTAGATCT1633.121409421677518Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1011.9341248563768672No Hit
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGTGT130.24894676369207203Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCTACAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCT90.17234775947912676Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGT80.15319800842589046Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)