FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB11LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB11LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158876
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATG68174.290767642689897TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTAT67264.233490269140713TruSeq Adapter, Index 22 (97% over 39bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCA10920.6873284825901961TruSeq Adapter, Index 22 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCGTC8790.5532616631838666TruSeq Adapter, Index 22 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCG6560.4129006269040006TruSeq Adapter, Index 22 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCA6550.41227120521664695TruSeq Adapter, Index 22 (97% over 39bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT3890.2448450363805735TruSeq Adapter, Index 9 (97% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCGTCTT3850.24232734963115887TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCGTCTTCT3700.232886024320854TruSeq Adapter, Index 22 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGC3630.22848007250937835TruSeq Adapter, Index 22 (97% over 38bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAC2900.18253228933256124TruSeq Adapter, Index 22 (97% over 38bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGC2320.146025831466049TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCAGCTCGTAT2070.1302902892822075TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCAGCTCGTATG1940.12210780734660993TruSeq Adapter, Index 22 (97% over 39bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCAT1830.11518416878571967TruSeq Adapter, Index 22 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)