FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB10LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB10LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34735
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG415311.95624010364186TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTAT22426.45458471282568TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCG4031.160213041600691TruSeq Adapter, Index 1 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGC2070.5959406938246725TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATATCGTATG1500.43184108248164677TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTC1480.4260832013818915TruSeq Adapter, Index 1 (96% over 31bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAA1440.41456743918238087TruSeq Adapter, Index 1 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAA1390.40017273643299267TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTCGTATG930.267741471138621TruSeq Adapter, Index 1 (97% over 36bp)
AGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTCTTCT930.267741471138621TruSeq Adapter, Index 1 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATATCGTAT840.24183100618972217TruSeq Adapter, Index 1 (97% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTCTT830.2389520656398445TruSeq Adapter, Index 1 (96% over 29bp)
GCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC690.19864689794155752TruSeq Adapter, Index 1 (97% over 36bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCA690.19864689794155752TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTCGTAT620.178494314092414TruSeq Adapter, Index 1 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCC620.178494314092414TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAAGCTCGTATG540.15546278969339283TruSeq Adapter, Index 1 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG540.15546278969339283TruSeq Adapter, Index 13 (97% over 35bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAAT490.1410680869440046TruSeq Adapter, Index 1 (97% over 36bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGC400.1151576219951058TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAAGCTCGTAT370.10652080034547287TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)