FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB09LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB09LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12143
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATG378731.186691921271514TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTAT193715.951577040270115TruSeq Adapter, Index 15 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATA3572.939965412171621TruSeq Adapter, Index 15 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGTC3172.6105575228526723TruSeq Adapter, Index 15 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCG2782.289384830766697TruSeq Adapter, Index 15 (97% over 34bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGAT2722.2399736473688545TruSeq Adapter, Index 15 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGC2131.7540970106234046TruSeq Adapter, Index 15 (97% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGTCTT1941.5976282631969034TruSeq Adapter, Index 15 (96% over 30bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATA1901.5646874742650085TruSeq Adapter, Index 15 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGTCTTCT1701.399983529605534TruSeq Adapter, Index 15 (96% over 28bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTC950.7823437371325043TruSeq Adapter, Index 15 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATA810.667050975870872TruSeq Adapter, Index 3 (97% over 36bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATG520.42823025611463394TruSeq Adapter, Index 15 (97% over 37bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTA480.395289467182739TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATATCGTATG460.37881907271679155TruSeq Adapter, Index 15 (97% over 37bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCT450.3705838754838178TruSeq Adapter, Index 15 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCC430.3541134810178704TruSeq Adapter, Index 15 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATG430.3541134810178704TruSeq Adapter, Index 1 (97% over 35bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGAGATCGGAAGAGCACA420.34587828378489666Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATAT410.33764308655192293TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA290.23882071975623817Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATGTCGTAT290.23882071975623817TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGGACACGTCTGAACTCCAGTCACATATGATATCTCGTATG290.23882071975623817TruSeq Adapter, Index 1 (97% over 35bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTA290.23882071975623817TruSeq Adapter, Index 15 (97% over 37bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTC280.23058552252326445TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATGTCGTATG250.20587993082434325TruSeq Adapter, Index 15 (97% over 37bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATAT250.20587993082434325TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACATATGATATCTCGTATGC250.20587993082434325TruSeq Adapter, Index 1 (95% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGT220.18117433912542205TruSeq Adapter, Index 15 (96% over 33bp)
TGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATAT200.16470394465947458TruSeq Adapter, Index 15 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGTCTTC200.16470394465947458TruSeq Adapter, Index 15 (96% over 29bp)
GCACACGTCTGAACTCCAGTCACATATGATATCTCGTATGCCGTCTTCTG190.15646874742650088TruSeq Adapter, Index 15 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATAGCTCGTATG190.15646874742650088TruSeq Adapter, Index 15 (97% over 37bp)
ACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCT170.1399983529605534TruSeq Adapter, Index 15 (97% over 37bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGA170.1399983529605534TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATATATCGTAT160.13176315572757968TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGATAGCTCGTAT140.11529276126163222TruSeq Adapter, Index 15 (97% over 37bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATA140.11529276126163222TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATATGATATCTCGTATG130.10705756402865849TruSeq Adapter, Index 1 (97% over 35bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACATATGATATCTCGTATG130.10705756402865849TruSeq Adapter, Index 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)