FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB08LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB08LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTAT110.3569110966904607TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATG100.32446463335496434TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGCGTATG70.22712524334847503TruSeq Adapter, Index 16 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAA50.16223231667748217TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)