FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB07LB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB07LB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG21615.766423357664234TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT15611.386861313868613TruSeq Adapter, Index 20 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC1128.175182481751825TruSeq Adapter, Index 20 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTC342.4817518248175183TruSeq Adapter, Index 20 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATA302.18978102189781TruSeq Adapter, Index 20 (97% over 38bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCAT221.6058394160583942TruSeq Adapter, Index 20 (97% over 38bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATA201.4598540145985401TruSeq Adapter, Index 20 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCG201.4598540145985401TruSeq Adapter, Index 20 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTT130.948905109489051TruSeq Adapter, Index 20 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTTCT130.948905109489051TruSeq Adapter, Index 20 (96% over 29bp)
GGGGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTC80.583941605839416TruSeq Adapter, Index 20 (96% over 30bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTC80.583941605839416TruSeq Adapter, Index 20 (97% over 38bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGC70.5109489051094891TruSeq Adapter, Index 20 (97% over 38bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT70.5109489051094891TruSeq Adapter, Index 9 (97% over 36bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCT70.5109489051094891TruSeq Adapter, Index 20 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG60.43795620437956206TruSeq Adapter, Index 21 (97% over 36bp)
GACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATC50.36496350364963503TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATATCGTATG50.36496350364963503TruSeq Adapter, Index 20 (97% over 38bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTC50.36496350364963503TruSeq Adapter, Index 20 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCC50.36496350364963503TruSeq Adapter, Index 20 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA40.291970802919708Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTA30.21897810218978103TruSeq Adapter, Index 20 (97% over 38bp)
CCAGTCACAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATAT30.21897810218978103TruSeq Adapter, Index 21 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATATCGTATGC30.21897810218978103TruSeq Adapter, Index 20 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGAGATCGGAAGAGCACA30.21897810218978103Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG30.21897810218978103TruSeq Adapter, Index 21 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGACGTCTGAACTCCAGTCACGTTGC30.21897810218978103TruSeq Adapter, Index 21 (95% over 23bp)
GATCGGAAGAGCACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCC30.21897810218978103TruSeq Adapter, Index 21 (95% over 24bp)
CAGATCGGAAGAGCACACGTCTGAGATCGGAAGAGCACACGTCTGAACTC20.145985401459854Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTTC20.145985401459854TruSeq Adapter, Index 20 (96% over 30bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC20.145985401459854TruSeq Adapter, Index 21 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATAGCTCGTAT20.145985401459854TruSeq Adapter, Index 20 (97% over 38bp)
CCCCTTCTGAAGCTTCTCCATGTTTGGATTGCGCAGAGATCGGAAGAGCA20.145985401459854No Hit
AGATCGGAAGAGCACACGTTCTGAACTCCAGTCACGTTGCATATCTCGTA20.145985401459854No Hit
GAAGAAGACTAGCTGAGCTCTCGGAAGAGCACACGTCTGAACTCCAGTCA20.145985401459854Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTA20.145985401459854TruSeq Adapter, Index 20 (97% over 38bp)
GAAGAAGACTAGCTGAGCTCTCGGAAGAGAGATCGGAAGAGCACACGTCT20.145985401459854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGAACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG20.145985401459854TruSeq Adapter, Index 21 (97% over 36bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC20.145985401459854TruSeq Adapter, Index 20 (96% over 29bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATAT20.145985401459854TruSeq Adapter, Index 20 (97% over 38bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGAGATCGGAAGA20.145985401459854Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCTACAAGTTTGGCAGAAAAGAGAAAAAATAGGGTGTAGTTCAAAAAGGC20.145985401459854No Hit
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT20.145985401459854TruSeq Adapter, Index 21 (97% over 36bp)
GGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGT20.145985401459854TruSeq Adapter, Index 20 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)