FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB07EB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB07EB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9997
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCGGTCATCTCGTATG210.2100630189056717TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCGGTCATCTCGTAT180.18005401620486144TruSeq Adapter, Index 6 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCGGTCA160.1600480144043213TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCGGTCATGTCGTAT130.13003901170351106TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCGGTCATGTCGTATG100.10003000900270081TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)