FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB06LB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB06LB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length151
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG7456.060606060606055TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTAT118.333333333333332TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATATCGTATG32.272727272727273TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTCGTATG21.5151515151515151TruSeq Adapter, Index 1 (97% over 36bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCACACGTCTGAA21.5151515151515151Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATC21.5151515151515151TruSeq Adapter, Index 1 (97% over 36bp)
TTATACTCTCTGAAATTTTCTCGGATTTTTCAGAGCTCAATTCTTAAATA10.7575757575757576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAAGCTCGTATG10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGACTGAACTCCAGTCACAGCGCCAATCTCGTATG10.7575757575757576TruSeq Adapter, Index 13 (97% over 35bp)
TGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATC10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
GCTGACGTACGTCCAAATGCATGGATTTATTGCAGATGTCCCTTAGATTT10.7575757575757576No Hit
ACCCTTCGTTCGCCATGATCTCGGTCTCGTCGAGGACCGTCACGAGATCC10.7575757575757576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCCAATCTCGTATG10.7575757575757576TruSeq Adapter, Index 5 (97% over 37bp)
AGTTGTTCAACTTTTTGACGGGCAACCATGGACTCTTTGATATATTCGCG10.7575757575757576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCGAATCTCGTATG10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC10.7575757575757576Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGCCAACCTTCCTGCGATTTTTGCCAAGGGTGCGCTGCTGTCCAAGAA10.7575757575757576No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGC10.7575757575757576TruSeq Adapter, Index 1 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG10.7575757575757576TruSeq Adapter, Index 13 (97% over 35bp)
AGGTTCCACAAACCGATGAACAACGAAACTGCAATGGTAAAAATAATCTA10.7575757575757576No Hit
GGACAGACAGTCCGGTTTGGCCGCCAACAAGGCCCGAACGGCGGAAGTAT10.7575757575757576No Hit
GCAGCTTAAACTGTCGGAATGGCGCGCGAGGAAATATTCGATCGGAAGAG10.7575757575757576No Hit
GTTTTGGAGCAGAGGCTTTTTTCTTGCTGAGCAGCCTTTCAAGTTATGTC10.7575757575757576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCCAATATCGTATG10.7575757575757576TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCGAGAAGATCGGAAGAGCACACGTCTGAACTCC10.7575757575757576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATATCGTATGC10.7575757575757576TruSeq Adapter, Index 1 (97% over 35bp)
TTGCAAAAAGGAGATGTATTATATGATGAGTATTATGGCAACGGTATCAT10.7575757575757576No Hit
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCA10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
TCTTGTAACCCTGAGTAGGTGTGTTGTTATCGTCCATCAGTATCCCACAG10.7575757575757576No Hit
GCAGAGCGTAAGCGTCAGAGCGTAAGCGGCAGAGCGTAAGCGGCAGAGCG10.7575757575757576No Hit
TTCTGATTTTCTTCTATGGCTGCTTGAGTAAGTAAATTTGCTAGATCGGA10.7575757575757576No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCAAATATCGTATGA10.7575757575757576TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTCGTAT10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
AGACTCCACCAGCACCCCATCCACGCCGCCGACCACGACAATCAAGGAGA10.7575757575757576No Hit
CCTTTACAAGTATGCGGTTATTCACTGCTTTCATAGCACATAGATCGGAA10.7575757575757576No Hit
GAAGAAATCCCCAAAAGTAAAAAGACACCTTTGCGGTAGTACACCTTCAG10.7575757575757576No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT10.7575757575757576Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCCAATCTCGTAT10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
GATCGTGCCCTCCCCGAACACATAAACGCAAAAATTGCTACCATCTACTA10.7575757575757576No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTA10.7575757575757576TruSeq Adapter, Index 1 (97% over 36bp)
GTGTTTGTGTGTGTGGATCACGGGACGAGACGATGTGTGCAGTTGCCGAG10.7575757575757576No Hit
TTGTACCTGCTCTAAGTATTACCTAATTTTTTTTCAAAAATGGATTATTT10.7575757575757576No Hit
CGATCGCCCTGCGCCGGCACCAGAGGGCCTTCGCTGAGTCGGAGCCAGCC10.7575757575757576No Hit
ATGGTCTCTTAAATAATTTAAGATTAGCTCCATATTAAACACTCAGCTTT10.7575757575757576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)