FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB05LB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB05LB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG3246.212847555129435TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT531.0162991371045063TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTAGCTCGTATG120.23010546500479384TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGC90.1725790987535954TruSeq Adapter, Index 18 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTA80.15340364333652923TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTC80.15340364333652923TruSeq Adapter, Index 18 (96% over 32bp)
GAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTCTTC60.11505273250239692TruSeq Adapter, Index 18 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)