FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB05EB_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB05EB_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5222
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATG83515.990042129452316TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTAT1552.9682114132516277TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATATCGTATG240.4595940252776714TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATGC140.2680965147453083TruSeq Adapter, Index 3 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAA120.2297970126388357TruSeq Adapter, Index 3 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATG120.2297970126388357TruSeq Adapter, Index 10 (97% over 35bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATC110.21064726158559938TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATGTCGTATG110.21064726158559938TruSeq Adapter, Index 3 (97% over 37bp)
GTTCTGTAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG80.15319800842589046TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)