FastQCFastQC Report
Fri 27 May 2022
HTFFKBGXL_n01_HRB0245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFFKBGXL_n01_HRB0245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981855
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTC227842.3205055736335813TruSeq Adapter, Index 23 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTAT71310.7262783201185511TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG28030.2854800352394193TruSeq Adapter, Index 23 (97% over 40bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA11340.11549566891241579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)