FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_zz_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_zz_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6817381
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT562600.8252435942776266TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG66300.040.5778411
ACACGTC67750.039.70938513
ACGTCTG68750.039.13179415
CACACGT69100.038.86991512
GATCGGA66450.038.610921
CACGTCT69900.038.55093414
CGTCTGA69950.038.36614216
CAGTCAC70250.037.98477627
AGCACAC70900.037.9761710
GAACTCC71650.037.36511221
CTCGTAT69650.037.2122744
TCCAGTC72000.037.12264325
CCAGTCA72200.036.9893426
GAGCACA73250.036.9680379
TATCTCG68500.036.9398241
GTCACAT73500.036.51656329
AGTCACA73550.036.34058428
AGAGCAC74800.036.2902158
GTCTGAA75200.035.8339117
TCTCGTA71050.035.798843
Produced by FastQC (version 0.11.2)