FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_zz_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_zz_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6956101
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT162570.2337085099828194TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25900.033.97272511
ACACGTC27000.032.83259213
CACACGT27850.031.8309812
GTCACAC27250.031.4053229
ACGTCTG28400.030.98172415
CACGTCT29100.030.38764214
GATCGGA28000.030.2580871
TATCTCG28000.030.17623941
CGTCTGA29650.029.97233616
AGCACAC30150.029.2568310
CACACTG29950.029.08935531
TCCAGTC31200.028.0619225
CCAGTCA31500.027.79506526
GAACTCC31950.027.74645421
CAGTCAC31250.027.73584427
CTCGTAT31150.027.12315244
AGTCACA31950.027.05931728
GAGCACA33400.026.6736159
TCACACT33350.026.3216330
TCTCGTA32050.026.29455443
Produced by FastQC (version 0.11.2)