FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah9171i3h-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah9171i3h-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7171646
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC304780.4249791470465776TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG41750.037.15224533
AGTCACG42450.036.8454428
GCACACG43000.036.78138411
GTCACGA42350.036.4669829
CACGATC43200.036.10618631
CACGTCT45150.035.12708714
ACGTCTG45050.035.00974315
GATCGGA42900.034.8817251
ACACGTC45450.034.79842813
GATCTCG38950.034.46087339
CGTCTGA45950.034.4197716
CAGTCAC45650.034.2626327
CACACGT46100.034.2125812
CGTATGC46000.033.4810244
AGCACAC47600.033.2268810
TCCAGTC47350.032.9858125
GAACTCC48200.032.76899721
GAGCACA48550.032.622029
CTCGTAT45350.032.50747742
CCAGTCA48400.032.49769226
Produced by FastQC (version 0.11.2)