FastQCFastQC Report
Thu 9 Nov 2017
HTCTCAFXX_n01_ah9171c3htop2-1117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTCTCAFXX_n01_ah9171c3htop2-1117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7696346
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC170040.220936013011889TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29150.033.73206311
ACGTCTG29950.032.610715
ACACGTC30800.031.78214813
GATCGGA30100.031.436281
CACTAGC31850.030.94531
CACACGT31650.030.928612
CACGTCT32000.030.65905814
CGTCTGA32700.029.80074316
AGCACAC33400.029.7036410
TCCAGTC33000.029.66391625
GAGCACA34100.029.1583969
CAGTCAC33900.028.94145827
CGTATGC32750.028.88807344
ACTAGCT34500.028.63183232
CCAGTCA34750.028.2968426
AGAGCAC35400.027.9631468
GAACTCC35750.027.56649421
TATCTCG32100.027.55636839
TCACTAG36850.027.04455230
CTCGTAT34050.026.62464742
Produced by FastQC (version 0.11.2)